Annealing Primers For Ligation at Eleanor Cooper blog

Annealing Primers For Ligation. for the purposes of this tutorial we will discuss how to add new restriction sites to the mcs of an empty vector.  — this protocol describes how to anneal and phosphorylate oligos to add short sequences to plasmids via ligation. for trickier ligations (such as ligation of annealed oligos) the efficiency of ligation can be improved by incubation at 37°c. Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng of vector per annealing. Digest vector with the appropriate restriction enzymes.

PPT MOLECULAR PowerPoint Presentation, free download ID1853398
from www.slideserve.com

for trickier ligations (such as ligation of annealed oligos) the efficiency of ligation can be improved by incubation at 37°c.  — this protocol describes how to anneal and phosphorylate oligos to add short sequences to plasmids via ligation. Digest vector with the appropriate restriction enzymes. for the purposes of this tutorial we will discuss how to add new restriction sites to the mcs of an empty vector. Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng of vector per annealing.

PPT MOLECULAR PowerPoint Presentation, free download ID1853398

Annealing Primers For Ligation  — this protocol describes how to anneal and phosphorylate oligos to add short sequences to plasmids via ligation. for trickier ligations (such as ligation of annealed oligos) the efficiency of ligation can be improved by incubation at 37°c.  — this protocol describes how to anneal and phosphorylate oligos to add short sequences to plasmids via ligation. for the purposes of this tutorial we will discuss how to add new restriction sites to the mcs of an empty vector. Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng of vector per annealing. Digest vector with the appropriate restriction enzymes.

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